Sorbitol dehydrogenase (SDH)

Sorbitol dehydrogenase (SDH), Calibrator, Controls and Linearity Checkset

Reagents for the determination of sorbitol dehydrogenase in blood serum

Sorbitol dehydrogenase (SDH), also known as Iditol dehydrogenase, is a liver enzyme that has historically been the enzyme of choice when assaying horse serum to detect liver damage or acute hepatic anoxia.  In larger animals AST (Aspartate amino transferase) and LDH (Lactate dehydrogenase) are generally regarded as unreliable when the liver is under study.

SDH, although a relatively unstable enzyme when compared to AST or LDH, is a reliable marker in monitoring hepatocellular injury in not only horses but also in pigs and cattle when sampling and sample processing are conducted within a five-hour window.

The Catachem SDH assay is a two-reagent system in which fructose in the presence of NADH is enzymatically converted by SDH to sorbitol with the oxidation of NADH to NAD. The rate of decrease in Optical Density (OD) of the NADH at 340 nm is directly proportional to the enzymatic activity of the SDH enzyme.

The assay itself is very stable with improved packaging designed to increase its shelf life over previous versions of the product. Applications are available for most common automated chemistry systems.

Calibrator, Controls and the Linearity Checkset are lyophilized products.

Sorbitol dehydrogenase (SDH) assay – C432-0D – 3 x 50 mL + 3 x 10 mL

Sorbitol dehydrogenase Calibrator – C434-40 – 3 x 1 mL (Lyophilized)

 Sorbitol dehydrogenase Control 1 – C434-41 – 3 x 1 mL (Lyophilized)

 Sorbitol dehydrogenase Control 2 – C434-42 – 3 x 1 mL (Lyophilized)

Sorbitol dehydrogenase Linearity Checkset – C434-0L – 5 x 1 mL (Lyophilized)